Paper

Progress and problems in evaluating and validating alternative assays in toxicology.

Stark, D.M., Shopsis, C., Borenfreund, E., Babich, H.

Abstract

Stark, D.M., Shopsis, C., Borenfreund, E., Babich, H., Progress and problems in evaluating and validating alternative assays in toxicology. Fd Chem Toxic. 1986; 24: 449-455.

Various techniques have been proposed and subsequently developed as potential replacements for whole-animal topical toxicology assays. Comparisons of in vitro and in vivo endpoints, the solubility of test agents, solvent effects, interactions between components of complex test samples and the standardization of data presentation are factors that influence not only individual laboratory results but interlaboratory reproducibility and the acceptance of newly developed alternative methods. Representative alternative test systems for evaluating topical toxicity are discussed. Examples of problems, data and solutions based on the authors' experience and on experience in other laboratories are reviewed. Specific problems in choosing endpoints, comparing results, recognizing responses of different types of target cell, evaluating the influences of support media in culture and interpreting published animal-based data are included.

Need expert advice?

Talk to the people in the know by contacting one of our Veterinary experts.
Contura Vet brings together the combined experience of bioengineering and veterinary expertise in the development of products for the treatment of osteoarthritis in animals.
Enquire now
General enquires
Email: Info@conturavet.com | Phone: +44 (0) 845 862 3292